Total Skin Rejuvenation & Revitalisation Program (Vegetal Placenta)

AVAILABLE IN
NORMAL STRENGTH or TRIPLE STRENGTH

- A set of 10 ampoules x 4ml (Normal strength)
- A set of 10 ampoules x 5ml (New 2009 triple strength)

 

In November 2007, Lab Dom AVMM (Suisse) Inc. (No. 676998), a Swiss incorporated company, pioneers in Research & Development specialising indepth in Live Cell Therapy & Placental Therapy, went a step further by introducing an additional Vegetal Placenta in 'Cosmeceutical Form' to complement its existing 'Supplement Form' (VP Softgels) range. This New Cosmeceutical Range researched, developed, manufactured and approved in Switzerland under Swiss GMP stringent standards under the name of of Switzerland Total Skin Rejuvenation & Revitalisation Program.

of Switzerland Total Skin Rejuvenation & Revitalisation Program (Vegetal Placenta) or VP TSRRP, in short, is manufactured according to an exclusive process, using state-of-the art molecular fractionation technology. The process is performed at controlled temperature with sophisticated ultrafiltration units to allow for the selection of specific molecules and to ensure the maintenance of their potency. The manufacturing steps are performed in accordance with the current provisions for Swiss Good Manufacturing Practice (GMP), ensuring a very high quality product.



The role of Matrix Metalloproteinases (MMPs) in of Switzerland VP

Example of clinical studies

Proteins such as collagen, elastin and other are linked with others macromolecules such as glycosaminoglycans to form the extracellular matrix (ECM) components of all solid body tissues. Examples include cartilage, the fibrous sheaths of muscles, tendons and ligaments, and the dermal layer of the skin. The ECM components are in constant renewal, or turnover, a process based on an equilibrium between synthesis and degradation.

Matrix Metalloproteinase (MMP)

Collagen fibers are synthesized by cells such as skin fibroblast. In addition to their anabolic activity, chondrocytes and fibroblasts also produce and secrete enzymes known as collagenase or matrix metalloproteinase (MMPs). MMPs represent a special class of enzymes that target and cleave the fibrous proteins of the ECM. Almost paradoxically, chondrocytes and fibroblasts also produce inhibitors of MMPs. These inhibitors are important in restraining the degradative action of MMPs. A balance must therefore exist between these two opposite cellular activities in order to maintain or restore tissues matrix integrity.

In addition to their role in maintaining the integrity of the skin and other tissues, MMPs have also been shown to be involved in angiogenesis.

With ageing and in various conditions, the metabolic activity of cells such as fibroblasts may become disturbed. Such a disturbance may affect the equilibrium between production and breakdown of extra cellular matrice (Murphy and Reynolds, 1993). In these conditions, the balance between MMPs and inhibitor of MMPs is no longer respected, so that, in most cases, the enzymatic activity of MMPs is predominant. Ageing and long term sunlight exposure are all factors associated with an increased enzymatic activity of MMPs (Fisher, 1997). This activity eventually leads to the degradation of the collagen fibre meshwork.

Imprinted silicones showing Total Skin Rejuvenation & Revitalisation Program
(Vegetal Placenta) before/after 3 weeks

Subject A

Before Treatment	After Treatment

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Subject B

Before Treatment	After Treatment

Biological activities

Age-related degradation of the extra-cellular matrix is closely associated with skin wrinkling and sagging. The effects of age-related increase in the activity of MMPs and collagenases is most visible at the level of skin.

In addition, the premature appearance of wrinkles and sagging are linked to long-term sunlight exposure (Fisher, 1997) and cigarette smoking (Kadunce, 1991).

Ageing is a natural inevitable process. However, the damages caused by age-related degeneration of extra-cellular matrix could be modulated if attempts are made to reestablish and maintain the equilibrium between extracellular matrix production and enzymatic activity of MMPs.

TSRRP (VP) has some whitening properties on Pigmentation

Total Skin Rejuvenation & Revitalisation Program (Vegetal Placenta) or VP is a composition that will work as anti-MMPs, consolidating the ECM and amino acid that will be used to produce new proteins as collagen in the very specific way skin is organised.

Keratinocyte is the major cell type of the epidermis, making up about 90% of epidermal cells. The epidermis is divided into four or five layers (depending on skin type) based on keratinocyte morphology:

• Stratum basale (at the junction with the dermis)
  
• Stratum spinosum
  
• Stratum granulosum
  
• Stratum lucidum (only present in thick skin - i.e. palms of hand and soles of feet)
  
• Stratum corneum
  

Keratinocytes originate in the basal layer from the division of keratinocyte stem cells. They are pushed up through the layers of the epidermis, undergoing gradual differentiation until they reach the stratum corneum where they form a layer of enucleated, flattened, highly keratinized cells called squamous cells. This layer forms an effective barrier to the entry of foreign matter and infectious agents into the body and minimises moisture loss.

Keratinocytes are shed and replaced continuously from the stratum corneum. The time of transit from basal layer to shedding is approximately one month although this can be accelerated in conditions of keratinocyte hyperproliferation such as psoriasis.

The stratum corneum ("the horny layer") is the outermost layer of the epidermis (the outermost layer of the skin). It is composed mainly of dead cells that lack nuclei. As these dead cells slough off, they are continuously replaced by new cells from the stratum germinativum (basale). In the human forearm, for example, about 1300 cells/cm2/hr are shed and commonly accumulate as house dust. Cells of the stratum corneum contain keratin, a protein that helps keep the skin hydrated by preventing moisture evaporation. In addition, these cells can also absorb moisture, further aiding in hydration and explaining why humans and other animals experience wrinkling of the skin.

The stratum lucidum (Latin for "clear layer") is a thin, clear layer of skin cells in the epidermis, and is named for its translucent appearance under a microscope. The keratinocytes of the stratum lucidum do not feature distinct boundaries and are filled with eleidin, an intermediate form of keratin.

The stratum granulosum. This layer typically contains 1 to 3 rows of squamous cells with many small basophilic granules in their cytoplasm. These

Keratohyalin granules are a step in the synthesis of the waterproofing protein keratin, and contain large amounts of filaggrin. This is the highest layer in the epidermis where living cells are found, the stratum lucidum above appears clear due to auto-digestion of cellular organelles. This layer also includes lamellar granules and tonofibrils.

In the skin, the stratum spinosum is a multi-layered arrangement of cuboidal cells that sits beneath the stratum granulosum. Adjacent cells are joined by desmosomes giving them the spiny appearance from which their name is derived. Their nuclei are often darkened, which is an early sign of cell death. Their fate is sealed because the nutrients and oxygen in interstitial fluid have become exhausted before the fluid is able to reach them by diffusion. Cells of the stratum spinosum actively synthesize intermediate filaments called cytokeratins which are composed of keratin. These intermediate filaments are anchored to the desmosomes joining adjacent cells to provide structural support, helping the skin resist abrasion.

Stratum germinativum (also stratum basale or basal cell layer) is the layer of keratinocytes that lies at the base of the epidermis immediately above the dermis. It consists of a single layer of tall, simple columnar epithelial cells lying on a basement membrane. These cells undergo rapid cell division, mitosis to replenish the regular loss of skin by shedding from the surface. About 25% of the cells are melanocytes, which produce melanin which provides pigmentation for skin and hair.

Under this epiderm, is the derm: The dermis is a layer of skin beneath the epidermidis that consists of connective tissue and cushions the body from stress and strain. The dermis is tightly connected to the epidermis by a basement membrane. It also harbors many nerve endings that provide the sense of touch and heat. It particularly contains more blood vessels. The dermis is structurally divided into two areas: a superficial area adjacent to the epidermis, called the papillary region, and a deep thicker area known as the reticular region, which receives its name from the dense concentration of collagenous, elastic, and reticular fibers that weave throughout it. These protein fibers give the dermis its properties of strength, extensibility, and elasticity. For example, tattoo ink is injected into the dermis; stretch marks from pregnancy are also located in the dermis.



ECM (Extra Cellular Matrix)

All thoses cells are weltering in the Extra Cellular Matrix. In biology, extracellular matrix (ECM) is any material part of a tissue that is not part of any cell. Extracellular matrix is the defining feature of connective tissue.

The ECM's main components are various glycoproteins, proteoglycans and hyaluronic acid. In most animals, the most abundant glycoproteins in the ECM are collagens. In fact, collagen is the most abundant protein in the human body. ECM also contains many other components: proteins such as fibrin, elastin, fibronectins, laminins, and nidogens, and minerals such as blood plasma or serum with secreted free flowing antigens.

In addition it sequesters a wide range of cellular growth factors, and acts as a local depot for them. Changes in physiological conditions can trigger protease activities that cause local release of such depots. This allows the rapid and local activation of cellular functions, without de novo synthesis.

Given this diversity, ECM can serve many functions, such as providing support and anchorage for cells, providing a way of separating the tissues, and regulating intercellular communication. The ECM regulates a cell's dynamic behavior.



MMP (Matrix metalloproteinases)

MMPs are capable of degrading all kinds of extracellular matrix proteins, but also can process a number of bioactive molecules. MMPs are also thought to play a major role on cell behaviors such as cell proliferation, migration (adhesion/dispersion), differentiation, angiogenesis. They are distinguished from other endopeptidases by their ability to degrade extracellular matrix, and their specific evolutionary DNA Sequence.